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These results, including reduced affinity between Arg74Ala ubiquitin and monomeric KLHDC3-EloB/C, are consistent with prior results from a GPS-peptidome screen for KLHDC3-dependent degron sequences revealing a preference for Arg residues located at positions −2 through −6 of the degron 13

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doi: 10.1038/s41467-020-17064-0 8 DaiSMLiFJLongHZZhouZWLuoHYXuSGet al