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For silencing FOXO4, specific small interfering RNA (siRNA) was employed, with a negative control siRNA, and transfection was conducted using Lipofectamine 3000 [2]

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3.4 Changes in serum homocysteine by supplementation route and dosage Analysis of 10 comparisons showed that vitamin B12 supplementation was associated with a mean reduction of −35.8% in serum homocysteine concentrations (Supplementary Table S5)

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